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p-bluescript ii ks+ plasmid  (Agilent technologies)


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    Structured Review

    Agilent technologies p-bluescript ii ks+ plasmid
    P Bluescript Ii Ks+ Plasmid, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-bluescript+ii+ks++plasmid/pmc01079787-213-14-18
    Average 90 stars, based on 1 article reviews
    p-bluescript ii ks+ plasmid - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Expressing:

    Article Title: Cytoplasmic expression of antibodies, antibody fragments and antibody fragment fusion proteins in
    Article Snippet: .. Construction of a Dicistronic Expression Vector Without Signal Sequences A) Cloning the E. coli β-glucuronidase from E. coli RR1: The DNA sequence encoding E. coli β-glucuronidase was amplified by PCR from the E. coli strain RR1 using the primers E.c.β Gluc. for (AAG CTT TCA TTG TTT GCC TCC CTG CTG CGG SEQ ID NO: 1) and E.c.β Gluc. back (TCT AGA CCA TGG TAC GTC CTG TAC AAA CCC CA SEQ ID NO: 2), and cloned into the vector P bluescript II KS (Stratagene, La Jolla, Calif.) by way of the Xba I and Hind III sites (FIG. 1). ..

    Cloning:

    Article Title: Cytoplasmic expression of antibodies, antibody fragments and antibody fragment fusion proteins in
    Article Snippet: .. Construction of a Dicistronic Expression Vector Without Signal Sequences A) Cloning the E. coli β-glucuronidase from E. coli RR1: The DNA sequence encoding E. coli β-glucuronidase was amplified by PCR from the E. coli strain RR1 using the primers E.c.β Gluc. for (AAG CTT TCA TTG TTT GCC TCC CTG CTG CGG SEQ ID NO: 1) and E.c.β Gluc. back (TCT AGA CCA TGG TAC GTC CTG TAC AAA CCC CA SEQ ID NO: 2), and cloned into the vector P bluescript II KS (Stratagene, La Jolla, Calif.) by way of the Xba I and Hind III sites (FIG. 1). ..

    Sequencing:

    Article Title: Cytoplasmic expression of antibodies, antibody fragments and antibody fragment fusion proteins in
    Article Snippet: .. Construction of a Dicistronic Expression Vector Without Signal Sequences A) Cloning the E. coli β-glucuronidase from E. coli RR1: The DNA sequence encoding E. coli β-glucuronidase was amplified by PCR from the E. coli strain RR1 using the primers E.c.β Gluc. for (AAG CTT TCA TTG TTT GCC TCC CTG CTG CGG SEQ ID NO: 1) and E.c.β Gluc. back (TCT AGA CCA TGG TAC GTC CTG TAC AAA CCC CA SEQ ID NO: 2), and cloned into the vector P bluescript II KS (Stratagene, La Jolla, Calif.) by way of the Xba I and Hind III sites (FIG. 1). ..

    Amplification:

    Article Title: Cytoplasmic expression of antibodies, antibody fragments and antibody fragment fusion proteins in
    Article Snippet: .. Construction of a Dicistronic Expression Vector Without Signal Sequences A) Cloning the E. coli β-glucuronidase from E. coli RR1: The DNA sequence encoding E. coli β-glucuronidase was amplified by PCR from the E. coli strain RR1 using the primers E.c.β Gluc. for (AAG CTT TCA TTG TTT GCC TCC CTG CTG CGG SEQ ID NO: 1) and E.c.β Gluc. back (TCT AGA CCA TGG TAC GTC CTG TAC AAA CCC CA SEQ ID NO: 2), and cloned into the vector P bluescript II KS (Stratagene, La Jolla, Calif.) by way of the Xba I and Hind III sites (FIG. 1). ..

    Polymerase Chain Reaction:

    Article Title: Cytoplasmic expression of antibodies, antibody fragments and antibody fragment fusion proteins in
    Article Snippet: .. Construction of a Dicistronic Expression Vector Without Signal Sequences A) Cloning the E. coli β-glucuronidase from E. coli RR1: The DNA sequence encoding E. coli β-glucuronidase was amplified by PCR from the E. coli strain RR1 using the primers E.c.β Gluc. for (AAG CTT TCA TTG TTT GCC TCC CTG CTG CGG SEQ ID NO: 1) and E.c.β Gluc. back (TCT AGA CCA TGG TAC GTC CTG TAC AAA CCC CA SEQ ID NO: 2), and cloned into the vector P bluescript II KS (Stratagene, La Jolla, Calif.) by way of the Xba I and Hind III sites (FIG. 1). ..

    Countercurrent Chromatography:

    Article Title: Cytoplasmic expression of antibodies, antibody fragments and antibody fragment fusion proteins in
    Article Snippet: .. Construction of a Dicistronic Expression Vector Without Signal Sequences A) Cloning the E. coli β-glucuronidase from E. coli RR1: The DNA sequence encoding E. coli β-glucuronidase was amplified by PCR from the E. coli strain RR1 using the primers E.c.β Gluc. for (AAG CTT TCA TTG TTT GCC TCC CTG CTG CGG SEQ ID NO: 1) and E.c.β Gluc. back (TCT AGA CCA TGG TAC GTC CTG TAC AAA CCC CA SEQ ID NO: 2), and cloned into the vector P bluescript II KS (Stratagene, La Jolla, Calif.) by way of the Xba I and Hind III sites (FIG. 1). ..

    Clone Assay:

    Article Title: Cytoplasmic expression of antibodies, antibody fragments and antibody fragment fusion proteins in
    Article Snippet: .. Construction of a Dicistronic Expression Vector Without Signal Sequences A) Cloning the E. coli β-glucuronidase from E. coli RR1: The DNA sequence encoding E. coli β-glucuronidase was amplified by PCR from the E. coli strain RR1 using the primers E.c.β Gluc. for (AAG CTT TCA TTG TTT GCC TCC CTG CTG CGG SEQ ID NO: 1) and E.c.β Gluc. back (TCT AGA CCA TGG TAC GTC CTG TAC AAA CCC CA SEQ ID NO: 2), and cloned into the vector P bluescript II KS (Stratagene, La Jolla, Calif.) by way of the Xba I and Hind III sites (FIG. 1). ..

    Plasmid Preparation:

    Article Title: Cytoplasmic expression of antibodies, antibody fragments and antibody fragment fusion proteins in
    Article Snippet: .. Construction of a Dicistronic Expression Vector Without Signal Sequences A) Cloning the E. coli β-glucuronidase from E. coli RR1: The DNA sequence encoding E. coli β-glucuronidase was amplified by PCR from the E. coli strain RR1 using the primers E.c.β Gluc. for (AAG CTT TCA TTG TTT GCC TCC CTG CTG CGG SEQ ID NO: 1) and E.c.β Gluc. back (TCT AGA CCA TGG TAC GTC CTG TAC AAA CCC CA SEQ ID NO: 2), and cloned into the vector P bluescript II KS (Stratagene, La Jolla, Calif.) by way of the Xba I and Hind III sites (FIG. 1). ..

    Article Title: Plants synthesizing a modified starch, a process for the generation of the plants, their use, and the modified starch
    Article Snippet: .. Bacterial Strains and Plasmids The E. coli strain DH5a (Bethesda Research Laboratories, Gaithersburgh, USA) was used for the Bluescript vector p Bluescript II KS (Stratagene) and for the pBinAR Hyg and pBinB33 Hyg constructs. ..

    Construct:

    Article Title: Plants synthesizing a modified starch, a process for the generation of the plants, their use, and the modified starch
    Article Snippet: .. Bacterial Strains and Plasmids The E. coli strain DH5a (Bethesda Research Laboratories, Gaithersburgh, USA) was used for the Bluescript vector p Bluescript II KS (Stratagene) and for the pBinAR Hyg and pBinB33 Hyg constructs. ..

    other:

    Article Title: A New Yeast Poly(A) Polymerase Complex Involved in RNA Quality Control
    Article Snippet: The PCR product was subcloned into the NdeI and SalI restriction sites of the p-Bluescript II KS+ plasmid (Stratagene, La Jolla, California, United States).



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    90
    Agilent technologies p-bluescript ii ks+ plasmid
    P Bluescript Ii Ks+ Plasmid, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p-bluescript+ii+ks++plasmid/pmc01079787-213-14-18
    Average 90 stars, based on 1 article reviews
    p-bluescript ii ks+ plasmid - by Bioz Stars, 2026-10
    90/100 stars
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